Inhibition of HIV-1 replication by ribozymes that show poor activityin vitro
نویسندگان
چکیده
منابع مشابه
Target sequence-specific inhibition of HIV-1 replication by ribozymes directed to tat RNA.
The structural motif formed between a hammerhead ribozyme and its substrate consists of three RNA double helices in which the sequence 5' to the XUY is termed helix I and the sequence 3' to the XUY helix III. Two hammerhead ribozymes targeted to the tat gene of HIV-1SF2 were designed to study target specificity and the potential effect of helix I mismatch on ribozyme efficacy both in vitro and ...
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Viruses often use host machinery in unusual ways to execute different steps during their replication. To identify host factors critical for virus replication, we screened cDNA expression libraries for genes or gene fragments that could interfere with HIV-1 vector transduction. The DNA clone that most potently inhibited HIV-1 expression encoded the N-terminal 91 aa of the eukaryotic initiation f...
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Herein we describe a mutant of the two-exon HIV-1 Tat protein, termed Nullbasic, that potently inhibits multiple steps of the HIV-1 replication cycle. Nullbasic was created by replacing the entire arginine-rich basic domain of wild type Tat with glycine/alanine residues. Like similarly mutated one-exon Tat mutants, Nullbasic exhibited transdominant negative effects on Tat-dependent transactivat...
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Jun Ohkawa1,2,5, Noriko Yuyama2, Shiori Koseki1,2,5, Yutaka Takebe3, Matthias Homann4, Georg Sczakiel4 and Kazunari Taira1,2,5,* 1National Institute for Advanced Interdisciplinary Research, 1-1-4 Higashi, Tsukuba Science City 305-8562, Japan; 2National Institute of Bioscience and Human Technology, 1-1 Higashi, Tsukuba Science City 305-8566, Japan; 3AIDS Research Center, National Institute of In...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.22.5251